Endocrine Abstracts

Estrogen Receptor (ER) is the defining feature of luminal breast cancers, where is functions as a transcription factor. ER requires associated proteins to interact with the DNA, including the pioneer factors FoxA1 and GATA3, both of which mediate where in the genome ER resides. In the absence of FoxA1, ER binding and transcriptional activity is diminished, even in endocrine resistant contexts. We have utilized ChIP-seq in primary tumor material, coupled with functional analysi...

Estrogen receptor (ER) is the defining feature of luminal breast cancers, where it functions as a transcription factor. ER requires associated proteins to interact with the DNA, including the pioneer factors FoxA1 and GATA3, both of which mediate where in the genome ER resides. In the absence of FoxA1, ER binding and transcriptional activity is diminished, even in endocrine resistant contexts. We have utilized ChIP-seq in primary tumor material, coupled with functional analysi...

Estrogen receptor (ER) is the defining feature of luminal breast cancers, where is functions as a transcription factor in response to the ligand estrogen. The traditional view of ER getting recruited to promoters of target genes is too simplistic. The recent discovery of ER–DNA interaction regions from ER+ breast cancer cell lines has revealed that ER rarely associates with promoter regions of target genes and instead associates with enhancer elements significant distance...

Estrogen receptor (ER) is the defining feature of luminal breast cancers, where is functions as a transcription factor in response to the ligand estrogen. The traditional view of ER getting recruited to promoters of target genes is too simplistic. The recent discovery of ER-DNA interaction regions from ER+ breast cancer cell lines has revealed that ER rarely associates with promoter regions of target genes and instead associates with enhancer elements significant distances fro...

Understanding transcription factor activity is critical for defining mechanisms of gene regulation in disease. The traditional view of transcription factors getting recruited to promoters of target genes is too simplistic. We now know that many transcription factors regulate genes from enhancers or cis-regulatory elements that are in some cases, significant distances from the target gene. Furthermore, we know that much of the human genome can be transcribed under one condition...

Estrogen Receptor (ER) regulation of target gene transcription is a significant factor in tumor development and progression. Previous work on ER-mediated transcription focused primarily on promoter regions of target genes, although recent work from our lab combining ER Chromatin Immunoprecipitation (ChIP) with tiled microarrays covering chromosomes 21 and 22, showed that distal enhancers appear to be the primary ER binding sites, and that these sites require the Forkhead prote...

Glucuronidation is an enzymatic process that terminally inactivates steroid hormones, including estrogens and androgens, thereby influencing carcinogenesis in hormone-dependent cancers. While estrogens drive breast carcinogenesis via the estrogen receptor alpha (ERα), androgens play a critical role as prohormones for estrogen biosynthesis and ligands for the androgen receptor (AR). Herein, the expression and regulation of two androgen inactivating UDP-glucuronosyltransfer...